RKO-EGFP cell line

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RKO-EGFP Order

RKO-EGFP cell line
Catalog#
YC-C002-EGFP-P
Size
1*10^6
Transfection Culture Medium
Transfection Culture medium-A
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
RKO-EGFP cell line
Order

Detailed Product Information

Product category
EGFP Stable Cell Lines
cell name
RKO-EGFP
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Reporter gene
EGFP
Resistance gene
puro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Accession
CVCL_C8W5(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:RKO		 STR Info (Cell bank)
Cell Line:p53R [JHU-56]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11131113
D13S317 811811
D7S820 810810
D16S539 13131213
VWA 16221622
TH01 610610
AMEL XXXX
TPOX 11111111
CSF1PO 810810
D12S391 151920.0
FGA 202123.0
D2S1338 1616
D21S11 272930.0
D18S51 1112
D8S1179 91314.0
D3S1358 1619
D6S1043 1419
PENTAE 1113
D19S433 1414
PENTAD 1011
D1S1656 131417.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
EGFP validation
RKO-EGFP cell line EGFP validation

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the RKO cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a RKO-EGFP cell line that can maintain long-term, stable and high expression.
RKO-EGFP cell line
RKO-EGFP
Catalog#
YC-C002-EGFP-P
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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